hs738 st int cells  (ATCC)

Journal: Frontiers in Nutrition

Article Title: The postbiotic sodium butyrate synergizes the antiproliferative effects of dexamethasone against the AGS gastric adenocarcinoma cells

doi: 10.3389/fnut.2024.1372982

Figure Lengend Snippet: Cell growth inhibition (%) against the AGS gastric adenocarcinoma and cell viability (%) of the Hs 738.St/Int normal intestine cell lines at different concentrations of sodium butyrate (NaB), sodium propionate (NaP), magnesium acetate (MgA), and dexamethasone (Dex) for 72 h using the Alamar Blue assay ( n = 3).

Article Snippet: AGS (CRL-1739, ATCC) and HS738.St/Int cell lines (CRL-7869, ATCC) were purchased from the American Type Culture Collection (ATCC, United States).

Techniques: Inhibition, Alamar Blue Assay

Journal: Frontiers in Nutrition

Article Title: The postbiotic sodium butyrate synergizes the antiproliferative effects of dexamethasone against the AGS gastric adenocarcinoma cells

doi: 10.3389/fnut.2024.1372982

Figure Lengend Snippet: cell growth inhibition (%) against AGS gastric adenocarcinoma and cell viability (%) of Hs 738.St/Int normal intestine cell lines at different concentrations of Dex:NaB (2:8).

Article Snippet: AGS (CRL-1739, ATCC) and HS738.St/Int cell lines (CRL-7869, ATCC) were purchased from the American Type Culture Collection (ATCC, United States).

Techniques: Inhibition

Journal: Cancer Cell International

Article Title: Helicobacter pylori -induced fibroblast-derived Serpin E1 promotes gastric cancer growth and peritoneal dissemination through p38 MAPK/VEGFA-mediated angiogenesis

doi: 10.1186/s12935-023-03177-1

Figure Lengend Snippet: Co-injection of Hs738 overexpressing Serpin E1 and primary GC cells promotes peritoneal dissemination of cancer cells by promoting VEGFA expression and angiogenesis in nude mice. A Hs738 cells-derived Serpin E1 facilitates peritoneal dissemination of primary GC cells. Mice were killed 28 days after injection. B Quantitative analysis of tumor nodules in the peritoneal cavity by counting. C HE staining and IHC analysis of Ki67, Serpin E1, VEGFA, and CD31 on sections from the peritoneal tumor. Scale bars: 1 mm (left 2 columns) and 100 μm (right 3 columns). D Quantification of IHC staining using the Image J software (n = 9 tumor nodules per mouse). Bar graphs and dot plots show the mean ± SD, n = 5; ** p < 0.05, and *** p < 0.001

Article Snippet: The AGS human GC cell line (CRL-1739) and Hs738 human gastric fibroblast cell line (CRL-7869) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Injection, Expressing, Derivative Assay, Staining, Immunohistochemistry, Software

Journal: Cancer Cell International

Article Title: Helicobacter pylori -induced fibroblast-derived Serpin E1 promotes gastric cancer growth and peritoneal dissemination through p38 MAPK/VEGFA-mediated angiogenesis

doi: 10.1186/s12935-023-03177-1

Figure Lengend Snippet: Infection with H. pylori and Serpin E1 overexpression in fibroblasts promote the migration, tube formation, and VEGFA expression of HUVECs. A Chemotactic migration of HUVECs was assessed by Matrigel-coated Transwell assay, wherein HUVECs were seeded in the upper chamber while fibroblasts with and without H. pylori infection or their conditioned medium were added into the lower chamber. Scale bar = 200 μm. B Tube formation of HUVECs on Matrigel was assessed by a Transwell co-cultured model, where CAFs overexpressing Serpin E1 were added in the upper chamber and HUVECs were seeded in the Matrigel-covered lower chamber. Scale bar = 100 μm. C Western blotting analysis of VEGFA in HUVECs co-cultured with Hs738 cells overexpressing Serpin E1, directly and using a Transwell system. D Immunofluorescence staining of VEGFA in HUVECs co-cultured with Serpin E1-overexpressed Hs738 cells using a Transwell insert. Scale bars: 25 μm and 10 μm (bottom row). E VEGFA levels were determined in the culture supernatants of HUVECs directly and Transwell co-cultured with Serpin E1-overexpressed Hs738 cells by ELISA. Bar graphs show the mean ± SD (n = 3); * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.001

Article Snippet: The AGS human GC cell line (CRL-1739) and Hs738 human gastric fibroblast cell line (CRL-7869) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Infection, Over Expression, Migration, Expressing, Transwell Assay, Cell Culture, Western Blot, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Mitochondrial complex I inhibitors suppress tumor growth through concomitant acidification of the intra- and extracellular environment

doi: 10.1016/j.isci.2021.103497

Figure Lengend Snippet: Key resources table

Article Snippet: Hs738.St/Int (Hs738) human gastric stromal cells (CRL-7869) were obtained from the American Type Culture Collection.

Techniques: Recombinant, Synthesized, Staining, Isolation, Activity Assay, Lactate Assay, Software